|
|
|
|
|
| Tall, Amadou; Hervio-heath, Dominique; Teillon, Anna; Boisset-helbert, C.; Delesmont, R.; Bodilis, J.; Touron-bodilis, A.. |
| Aims To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37 degrees C on TCBS medium, in September 2009 from seawater and surface sediments. Methods and Results q-PCR assays previously selected for the identification of bacterial strains isolated at 37 degrees C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22 degrees C. The great majority of the 37 degrees C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85 center dot 9%) and V.harveyi (10 center dot 7%). The human pathogens V.parahaemolyticus and V.cholerae were rarely detected (two strains each).... |
| Tipo: Text |
Palavras-chave: Coastal environment; Culturable Vibrio spp; Diversity; PyrH and toxR genes partial sequencing. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25502/24832.pdf |
| |
|
|
| Tall, Amadou; Teillon, Anna; Boisset, Claire; Delesmont, R.; Touron-bodilis, A.; Hervio-heath, Dominique. |
| Aims: To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. Methods and Results: Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campaigns of environmental samples. Three real-time PCR assays were developed from a multiplex PCR, targeting V. vulnificus, V. cholerae and V. alginolyticus on the dnaJ gene. After testing their specificity, these systems were applied for the identification of 961 strains isolated at 22°C (446 strains) and 37°C (515 strains) in September 2009. The predominance of V.... |
| Tipo: Text |
Palavras-chave: DnaJ; Identification; Real-time PCR; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19901/17562.pdf |
| |
|
|
|
